Daniel V.T. Catenacci has held positions at the University of Chicago Medicine for over 15 years, starting as a fellow in Hematology and Medical Oncology from 2006 to 2010. He then joined the faculty at the University of Chicago as an Instructor in the Department of Medicine from 2010 to June 2012 before becoming an Assistant Professor of Medicine. In 2019, he became an Associate Professor of Medicine and held the position through January 2022.

For much of that time, Daniel V.T. Catenacci also worked at the University of Chicago Comprehensive Cancer Center as a Pharmacogenomics and Experimental Therapeutics member and later as an Assistant Director of Translational Research. He also worked as Associate Director of the Gastrointestinal Oncology Program and later the Interdisciplinary Director in the same area, where he oversaw the clinical and translational research enterprise for gastrointestinal cancers.