Kshitiz Singh


My primary professional (also a bit personal) relationship is with DNA. My relationship with DNA started after hearing the name of Laws of genetics (by Gregor Mendel), during early studies of science. While I was pursuing my Medical degree to become a doctor, this relationship became more closer, during the study of pathogenesis of diseases, primary genetic diseases, auto immune diseases and diseases of multifactorial inheritance. This made my imagination to look first time towards genome editing, when I was in second year of medicine. At that time, I dreamed about editing human genome, without possessing any access to hands-on DNA editing tool. But the dream continued and I thought that after completing the medicine degree, I will pursue research in human genetics.

In 2005, I got my license to work as physician in India, but the far-sight remained focused on human genetics. During this period, I got selected for the Interdisciplinary course for physicians to be trained as researchers, at Indian Institute of Technology, Kharagpur, West Bengal. This was my first stepping stone to get nearer to the DNA, the human genes. While attending this course, I joined for my Master's thesis at University of Fukui, Japan, under the guidance of Prof. Makoto Sato (http://www.researchgate.net/profile/Makoto_Sato9) and Prof. Kazuki Kuroda (http://www.researchgate.net/profile/Kazuki_Kuroda), with very friendly and expert advises from Prof. Tokuichi Iguchim(http://www.researchgate.net/researcher/71420173_Tokuichi_Iguchi).

This was the time that I began to feel the physical existence of DNA because I was seeing it every day in agarose gels, I was cutting it by using restriction enzymes, joining the two different DNA fragment by using ligases, sequencing the DNA and physically seeing each nucleotide appearing in the chromatogram of sequencing data. This led to enhancement in the curiosity and the hands-on knowledge in playing with DNA. During this period, while I was altering the DNA, primarily the plasmid DNA, I came across Zinc Finger Nucleases (ZFNs - marketed by Sigma Aldrich). This was very exciting as ZFNs were capable of altering human genomic DNA. This led to surge in excitement and extreme focus on editing genome. In my lifetime, I plan to use and help in harnessing genome editing capabilities of molecules such as TALENs & CRISPR/Cas for human health.

  • Work
    • Pondicherry Biotech Pvt. Ltd
  • Education
    • Indian Institute of Technology, Kharagpur, India
    • Vrije Universiteit Brussel
    • LLRM Medical College, Meerut, India